Enhanced Phototherapy by Nanoparticle-Enzyme via Generation and Photolysis of Hydrogen Peroxide

Chang, Kaiwen1,2; Liu, Zhihe3; Fang, Xiaofeng3; Chen, Haobin3; Men, Xiaoju3; Yuan, Ye3; Sun, Kai3; Zhang, Xuanjun2; Yuan, Zhen2; Wu, Changfeng1

2017-07

10.1021/acs.nanolett.7b01382

NANO LETTERS   影响因子和分区

1530-6984

1530-6992

Light has been widely used for cancer therapeutics such as photodynamic therapy (PDT) and photothermal therapy. This paper describes a strategy called enzyme-enhanced phototherapy (EEPT). for cancer treatment. We constructed a nanoparticle platform by covalent conjugation of glucose oxidase (GOx) to small polymer dots, which could be persistently immobilized into a tumor. While the malignant tumors have high glucose uptake, the GOx efficiently catalyzes the glucose oxidation with simultaneous generation of H2O2. Under light irradiation, the in situ generated H2O2 was photolyzed to produce hydroxyl radical, the most reactive oxygen species, for killing cancer cells. In vitro assays indicated that the cancer cells were destroyed by using a nanoparticle concentration at 0.2 mu g/mL and a light dose of similar to 120 J/cm(2), indicating the significantly enhanced efficiency of the EEPT method when compared to typical PDT that requires a photosensitizer of >10 mu g/mL for effective cell killing under the same light dose. Furthermore, remarkable inhibition of tumor growth was observed in xenograft-bearing mice, indicating the promise of the EEPT approach for cancer therapeutics.

Polymer dot glucose oxidase hydrogen peroxide hydroxyl radical enzyme-enhanced phototherapy

SCI ; EI

英语

NI期刊 ; NI期刊 ; NI论文

第一 ; 通讯

Key Program of National Natural Science Foundation of China[NSFC 61335001]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000405643300047

AMER CHEMICAL SOC

20172903962914

Cells ; Diseases ; Glucose ; Glucose sensors ; Hydrogen peroxide ; Nanoparticles ; Oxidation ; Peroxides ; Photodynamic therapy ; Photolysis ; Photosensitizers ; Tumors

Bioengineering and Biology:461 ; Biomedical Equipment, General:462.1 ; Light/Optics:741.1 ; Nanotechnology:761 ; Chemical Reactions:802.2 ; Organic Compounds:804.1 ; Inorganic Compounds:804.2 ; Solid State Physics:933

MATERIALS SCIENCE

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Univ Macau, Fac Hlth Sci, Taipa, Macau, Peoples R China
3.Jilin Univ, Coll Elect Sci & Engn, State Key Lab Integrated Optoelect, Changchun 130012, Jilin, Peoples R China

Chang, Kaiwen,Liu, Zhihe,Fang, Xiaofeng,et al. Enhanced Phototherapy by Nanoparticle-Enzyme via Generation and Photolysis of Hydrogen Peroxide[J]. NANO LETTERS,2017,17(7):4323-4329.

Chang, Kaiwen.,Liu, Zhihe.,Fang, Xiaofeng.,Chen, Haobin.,Men, Xiaoju.,...&Wu, Changfeng.(2017).Enhanced Phototherapy by Nanoparticle-Enzyme via Generation and Photolysis of Hydrogen Peroxide.NANO LETTERS,17(7),4323-4329.

Chang, Kaiwen,et al."Enhanced Phototherapy by Nanoparticle-Enzyme via Generation and Photolysis of Hydrogen Peroxide".NANO LETTERS 17.7(2017):4323-4329.