Light-Harvesting Fluorescent Spherical Nucleic Acids Self-Assembled from a DNA-Grafted Conjugated Polymer for Amplified Detection of Nucleic Acids

Xiao, Fan1,2; Fang, Xiaofeng3; Li, Hongyan2; Xue, Hanbing4; Wei, Zixiang2; Zhang, Wenkang2; Zhu, Yulin5; Lin, Li2; Zhao, Yan4; Wu, Changfeng3; Tian, Leilei2

2022-02-01

10.1002/anie.202115812

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   影响因子和分区

1433-7851

1521-3773

The ultralow concentration of nucleic acids in complex biological samples requires fluorescence probes with high specificity and sensitivity. Herein, a new kind of spherical nucleic acids (SNAs) is developed by using fluorescent pi-conjugated polymers (FCPs) as a light-harvesting antenna to enhance the signal transduction of nucleic acid detection. Specifically, amphiphilic DNA-grafted FCPs are synthesized and self-assemble into FCP-SNA structures. Tuning the hydrophobicity of the graft copolymer can adjust the size and light-harvesting capability of the FCP-SNAs. We observe that more efficient signal amplification occurs in larger FCP-SNAs, as more chromophores are involved, and the energy transfer can go beyond the Forster radius. Accordingly, the optimized FCP-SNA shows an antenna effect of up to 37-fold signal amplification and the limit of detection down to 1.7 pM in microRNA detection. Consequently, the FCP-SNA is applied to amplified in situ nucleic acid detecting and imaging at the single-cell level.

Light-Harvesting Antenna MicroRNA Detection Spherical Nucleic Acid pi-Conjugated Polymer

SCI ; EI

英语

NI期刊 ; NI论文

通讯

National Natural Science Foundation of China[51973089] ; Shenzhen Science and Technology Innovation Commission[KQTD20170810111314625]

Chemistry

Chemistry, Multidisciplinary

WOS:000750653500001

WILEY-V C H VERLAG GMBH

20220611602996

Antennas ; Biomolecules ; Chromophores ; Conjugated polymers ; Energy transfer ; Grafting (chemical) ; RNA ; Signal processing ; Signal transduction

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Information Theory and Signal Processing:716.1 ; Light/Optics:741.1 ; Biochemistry:801.2 ; Chemical Reactions:802.2 ; Organic Compounds:804.1 ; Organic Polymers:815.1.1

CHEMISTRY

Web of Science

1.Harbin Inst Technol, Sch Mat Sci & Engn, Harbin 150001, Heilongjiang, Peoples R China
2.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Guangdong, Peoples R China
3.Southern Univ Sci & Technol Inst, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen 518055, Guangdong, Peoples R China
5.Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Guangdong, Peoples R China

Xiao, Fan,Fang, Xiaofeng,Li, Hongyan,et al. Light-Harvesting Fluorescent Spherical Nucleic Acids Self-Assembled from a DNA-Grafted Conjugated Polymer for Amplified Detection of Nucleic Acids[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2022,61.

Xiao, Fan.,Fang, Xiaofeng.,Li, Hongyan.,Xue, Hanbing.,Wei, Zixiang.,...&Tian, Leilei.(2022).Light-Harvesting Fluorescent Spherical Nucleic Acids Self-Assembled from a DNA-Grafted Conjugated Polymer for Amplified Detection of Nucleic Acids.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,61.

Xiao, Fan,et al."Light-Harvesting Fluorescent Spherical Nucleic Acids Self-Assembled from a DNA-Grafted Conjugated Polymer for Amplified Detection of Nucleic Acids".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 61(2022).